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agei sali digested prk5 vector backbone  (New England Biolabs)


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    New England Biolabs agei sali digested prk5 vector backbone
    Agei Sali Digested Prk5 Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agei+sali+digested+prk5+vector+backbone/SalI/pm40468084-609-7-16
    Average 97 stars, based on 3240 article reviews
    agei sali digested prk5 vector backbone - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: To generate expression plasmids with eGFP–BRD4::NUT, the eGFP sequence was amplified from pRK5-eGFP-NPM1 plasmid described above and cDNA for BRD4::NUT fusion protein was amplified from Addgene plasmid 171630 (ref. 49) as two separate fragments, while generating KS and KSF-to-G fusions in the C terminus with reverse primers. .. BRD4::NUT and eGFP fragments were assembled into AgeI + SalI-digested pRK5 vector backbone (pRK5-mEGFP-HMGB1) using the NEBuilder HiFi DNA assembly master mix. ..

    Article Title: Probing condensate microenvironments with a micropeptide killswitch
    Article Snippet: To generate expression plasmids with eGFP–BRD4::NUT , the eGFP sequence was amplified from pRK5-eGFP-NPM1 plasmid described above and cDNA for BRD4::NUT fusion protein was amplified from Addgene plasmid 171630 (ref. ) as two separate fragments, while generating KS and KS F-to-G fusions in the C terminus with reverse primers. .. BRD4::NUT and eGFP fragments were assembled into AgeI + SalI-digested pRK5 vector backbone (pRK5-mEGFP-HMGB1) using the NEBuilder HiFi DNA assembly master mix. ..

    Expressing:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.

    Polymerase Chain Reaction:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.

    Introduce:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.

    Sequencing:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.

    Generated:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.

    Variant Assay:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: .. To generate these expression vectors, pRK5-mCherry-GFP-nb vector was PCR-amplified using primers that introduce HA tag before SV40-NLS, and an insert containing GSG-P2A-GSG-T2A sequence was generated as single-stranded oligonucleotide that was used to bridge double-stranded PCR-amplified pRK5-mCherry-GFP-nb vector using the NEBuilder HiFi DNA assembly master mix. pRK5-mCherry-P2AT2A-HA-tag-SV40-NLS-GFP-nb-1×KS, -2×KS, -1×KSF-to-A and -1×KSF-to-G vectors were generated by amplifying mCherry-P2A-T2A-HA-tag sequence from vector described above and GFP-nb variant sequences from previously prepared pRK5-mCherry-GFP-nb vectors, and fragments were assembled into AgeI + SalI-digested pRK5 vector backbone using the NEBuilder HiFi DNA assembly master mix. .. Vectors with 2×KSF-to-A and 2×KSF-to-G variants were generated by amplifying the vector with primers flanking the KS sequence and 2×KSF-to-A and 2×KSF-to-G sequences were generated as single-stranded oligonucleotide that was used to bridge double-stranded, PCR-amplified vector using the NEBuilder HiFi DNA assembly master mix.



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    agei sali digested prk5 vector backbone  (New England Biolabs)
    97
    New England Biolabs agei sali digested prk5 vector backbone
    Agei Sali Digested Prk5 Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agei+sali+digested+prk5+vector+backbone/SalI/pm40468084-609-7-16
    Average 97 stars, based on 1 article reviews
    agei sali digested prk5 vector backbone - by Bioz Stars, 2026-09
    97/100 stars
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